6GBS
Crystal Structure of the C. themophilum Scavenger Decapping Enzyme DcpS apo form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-03-13 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0004 |
Spacegroup name | P 1 |
Unit cell lengths | 52.595, 69.719, 70.947 |
Unit cell angles | 104.48, 101.39, 111.61 |
Refinement procedure
Resolution | 48.503 - 1.946 |
R-factor | 0.1845 |
Rwork | 0.183 |
R-free | 0.21650 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5bv3 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.870 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER (2.7.17) |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.503 | 48.503 | 2.000 |
High resolution limit [Å] | 1.946 | 8.720 | 1.950 |
Rmerge | 0.086 | 0.038 | 0.705 |
Rmeas | 0.102 | 0.045 | 0.834 |
Number of reflections | 115497 | 596 | 4454 |
<I/σ(I)> | 9.82 | 26.57 | 2.1 |
Completeness [%] | 96.3 | 85.8 | 95.8 |
Redundancy | 3.428 | 3.351 | 3.545 |
CC(1/2) | 0.996 | 0.996 | 0.746 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 200 mM Ammoniumacetate, 100 mM BisTRIS pH 5.5, 25 % PEG 3350 |