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6GB1

Crystal structure of the GLP1 receptor ECD with Peptide 11

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsSLS BEAMLINE X10SA
Synchrotron siteSLS
BeamlineX10SA
Temperature [K]100
Detector technologyPIXEL
Collection date2017-04-09
DetectorDECTRIS PILATUS 6M
Wavelength(s)0.999920
Spacegroup nameP 43 21 2
Unit cell lengths55.550, 55.550, 138.830
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution51.570 - 2.730
R-factor0.208
Rwork0.205
R-free0.26200
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)3c59
RMSD bond length0.010
RMSD bond angle1.180
Data reduction softwareXDS ((VERSION November 3)
Data scaling softwareautoPROC ((Version 1.1.6))
Phasing softwarePHASER
Refinement softwareBUSTER (2.11.7)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]51.5702.870
High resolution limit [Å]2.7302.730
Rmerge0.0751.096
Number of reflections6336
<I/σ(I)>222.3
Completeness [%]100.0100
Redundancy1212.8
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP7.5292A 6-fold excess of peptide 11 was dissolved in a solution of 12 mg/ml GLP1R-ECD in 10 mM Tris buffer pH 7.5, 100 mM NaSulfate and 2% glycerol. 100 nl of this protein solution plus 100 nl reservoir solution were equilibrated against reservoir solution consisting of 10 mM CoCl2, 9.4 % (v/v) 1,6-hexanediol and 100 mM NaAcetate, pH 4.8. Crystals appeared after about one week.

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