6G7K
Retinal isomerization in bacteriorhodopsin revealed by a femtosecond X-ray laser: 10 ps state structure
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | FREE ELECTRON LASER |
Source details | SLAC LCLS BEAMLINE CXI |
Synchrotron site | SLAC LCLS |
Beamline | CXI |
Temperature [K] | 298 |
Detector technology | PIXEL |
Collection date | 2017-06-30 |
Detector | CS-PAD CXI-1 |
Wavelength(s) | 1.3 |
Spacegroup name | P 63 |
Unit cell lengths | 62.320, 62.320, 111.100 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 18.091 - 1.900 |
R-factor | 0.2531 |
Rwork | 0.250 |
R-free | 0.29810 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5j7a |
RMSD bond length | 0.004 |
RMSD bond angle | 0.616 |
Data reduction software | CrystFEL (0.6.3) |
Data scaling software | CrystFEL (0.6.3) |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 18.091 | 1.990 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 18261 | |
<I/σ(I)> | 6.36 | |
Completeness [%] | 94.9 | |
Redundancy | 230.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 5.6 | 294 | 100 mM Na/K Phosphate buffer pH 5.6 30 % PEG 2000 |