Bacteriorhodopsin ground state structure obtained with Serial Femtosecond Crystallography

Summary for 5J7A

DescriptorBacteriorhodopsin, RETINAL, 1-[2,6,10.14-TETRAMETHYL-HEXADECAN-16-YL]-2-[2,10,14-TRIMETHYLHEXADECAN-16-YL]GLYCEROL, ... (4 entities in total)
Functional Keywordslight-driven proton pump, retinal binding, seven transmembrane helix protein, proton transport
Biological sourceHalobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1)
Total number of polymer chains1
Total molecular weight28341.51
Primary citation
Nogly, P.,Panneels, V.,Nelson, G.,Gati, C.,Kimura, T.,Milne, C.,Milathianaki, D.,Kubo, M.,Wu, W.,Conrad, C.,Coe, J.,Bean, R.,Zhao, Y.,Bath, P.,Dods, R.,Harimoorthy, R.,Beyerlein, K.R.,Rheinberger, J.,James, D.,DePonte, D.,Li, C.,Sala, L.,Williams, G.J.,Hunter, M.S.,Koglin, J.E.,Berntsen, P.,Nango, E.,Iwata, S.,Chapman, H.N.,Fromme, P.,Frank, M.,Abela, R.,Boutet, S.,Barty, A.,White, T.A.,Weierstall, U.,Spence, J.,Neutze, R.,Schertler, G.,Standfuss, J.
Lipidic cubic phase injector is a viable crystal delivery system for time-resolved serial crystallography.
Nat Commun, 7:12314-12314, 2016
PubMed: 27545823 (PDB entries with the same primary citation)
DOI: 10.1038/ncomms12314
MImport into Mendeley
Experimental method

Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers 0.21910 1.7% 6.2%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution
Download full validation reportDownload
PDB entries from 2020-10-21