6G6K

The crystal structures of Human MYC:MAX bHLHZip complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-14
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97950
Spacegroup name	P 1
Unit cell lengths	30.399, 52.310, 73.089
Unit cell angles	93.89, 101.92, 106.79

Refinement procedure

Resolution	38.266 - 1.350
R-factor	0.1743
Rwork	0.173
R-free	0.20260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1knp
RMSD bond length	0.005
RMSD bond angle	0.756
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.270	1.420
High resolution limit [Å]	1.350	1.350
Rmerge	0.062	0.438
Rmeas	0.079	0.559
Rpim	0.049	0.345
Number of reflections	80704
<I/σ(I)>	8.7	2.1
Completeness [%]	87.9	81.3
Redundancy	2.4	2.3
CC(1/2)	0.989	0.748

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	10% w/v PEG 8000, 20% v/v ethylene glycol, 0.075 %(w/v) of each Additive, 5 %(v/v) EtOH, 0.1 M MOPS/HEPES-Na, pH 7.5, Additive: 0.75 %(w/v) menthol, 0.75 %(w/v) caffeic acid, 0.75 %(w/v) D-quinic acid, 0.75 %(w/v) shikimic acid, 0.75 %(w/v) gallic acid monohydrate, 0.75 %(w/v) N-vanillylnonanamide.