6G63
RNase E in complex with sRNA RrpA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-10-01 |
Detector | DECTRIS EIGER R 4M |
Wavelength(s) | 0.987 |
Spacegroup name | P 61 |
Unit cell lengths | 110.630, 110.630, 466.020 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.947 - 3.950 |
R-factor | 0.2757 |
Rwork | 0.275 |
R-free | 0.29620 |
RMSD bond length | 0.005 |
RMSD bond angle | 1.012 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 95.800 | 4.190 |
High resolution limit [Å] | 3.950 | 3.950 |
Rmerge | 0.150 | |
Number of reflections | 28217 | |
<I/σ(I)> | 8.3 | |
Completeness [%] | 100.0 | |
Redundancy | 9.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.1 M KCl, 0.01 M MgCl2, 0.05 M Tris-HCl pH 8.5 and 30% (v/v) PEG 400 |