6G4Q
Structure of human ADP-forming succinyl-CoA ligase complex SUCLG1-SUCLA2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-11-29 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9795 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 130.200, 130.200, 119.772 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 65.100 - 2.590 |
R-factor | 0.2096 |
Rwork | 0.207 |
R-free | 0.25350 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1euc |
Data reduction software | xia2 |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 65.100 | 2.660 |
High resolution limit [Å] | 2.590 | 2.590 |
Number of reflections | 36946 | 2712 |
<I/σ(I)> | 14.4 | 2.1 |
Completeness [%] | 100.0 | 100 |
Redundancy | 9.7 | |
CC(1/2) | 0.999 | 0.547 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293.15 | 2.1M DL- malic acid |