6G38
Crystal structure of haspin in complex with tubercidin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-11-30 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 78.560, 78.760, 80.020 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.050 - 1.470 |
| R-factor | 0.14437 |
| Rwork | 0.143 |
| R-free | 0.16544 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ouc |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.537 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.050 | 1.450 |
| High resolution limit [Å] | 1.430 | 1.430 |
| Rmerge | 0.102 | |
| Number of reflections | 92144 | 4423 |
| <I/σ(I)> | 8.6 | 1.2 |
| Completeness [%] | 99.9 | 98.3 |
| Redundancy | 5.9 | 4.2 |
| CC(1/2) | 0.994 | 0.379 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277.15 | 51-63% MPD and 0.1M SPG buffer, pH 6.0-6.5 |
