6G2B
X-ray structure of NSD3-PWWP1 in complex with compound 8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-09-17 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.895, 47.439, 64.301 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.170 - 1.610 |
| R-factor | 0.2 |
| Rwork | 0.199 |
| R-free | 0.22500 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.900 |
| Data scaling software | Aimless (0.5.12) |
| Phasing software | BALBES |
| Refinement software | BUSTER (2.11.7) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.170 | 1.613 |
| High resolution limit [Å] | 1.607 | 1.607 |
| Rmerge | 0.046 | 0.944 |
| Rmeas | 0.050 | 0.824 |
| Rpim | 0.020 | 0.325 |
| Number of reflections | 18102 | 2609 |
| <I/σ(I)> | 23.9 | 1.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.4 | 6.2 |
| CC(1/2) | 1.000 | 0.817 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 277 | 100mM Morpheus Buffer 3, 30% P550MME_P20K, 10% Morpheus Ethylene glycols |
