6G0A
The crystal structure of the Pol2 catalytic domain of DNA polymerase epsilon carrying a P301R substitution.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-04 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9840 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 159.895, 67.252, 151.639 |
| Unit cell angles | 90.00, 111.50, 90.00 |
Refinement procedure
| Resolution | 19.977 - 2.620 |
| R-factor | 0.226 |
| Rwork | 0.224 |
| R-free | 0.26510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4m8o |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 78.780 | 78.780 | 2.710 |
| High resolution limit [Å] | 2.620 | 10.150 | 2.620 |
| Rmerge | 0.128 | 0.086 | 0.859 |
| Rmeas | 0.156 | 0.110 | 1.054 |
| Rpim | 0.087 | 0.068 | 0.601 |
| Total number of observations | 136067 | 2411 | 12302 |
| Number of reflections | 44773 | 830 | 4351 |
| <I/σ(I)> | 5.9 | 18.1 | 1.3 |
| Completeness [%] | 98.7 | 97.8 | 98.2 |
| Redundancy | 3 | 2.9 | 2.8 |
| CC(1/2) | 0.829 | 0.653 | 0.368 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 292 | 50mM MES pH 6.5, 150mM NaAc and 8% PEG20K |
