6FZT
Crystal structure of Smad8_9-MH1 bound to the GGCGC site.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE MASSIF-3 |
Synchrotron site | ESRF |
Beamline | MASSIF-3 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-12-04 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 0.967 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 75.433, 79.509, 88.372 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 59.110 - 2.460 |
R-factor | 0.195 |
Rwork | 0.193 |
R-free | 0.23700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6fzs |
RMSD bond length | 0.010 |
RMSD bond angle | 1.040 |
Data reduction software | XDS |
Data scaling software | autoPROC |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 59.110 | 2.630 |
High resolution limit [Å] | 2.460 | 2.460 |
Rpim | 0.040 | 0.550 |
Number of reflections | 15793 | 791 |
<I/σ(I)> | 14.3 | 1.3 |
Completeness [%] | 92.0 | 54.3 |
Redundancy | 6 | 6.3 |
CC(1/2) | 0.998 | 0.570 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 0.2M NaF, 0.1 M bis-tris propane pH 8.5, 20% PEG3350 |