6FZP
PPAR gamma complex.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-10-20 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.968 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 53.592, 86.268, 122.693 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 17.320 - 2.300 |
| R-factor | 0.219 |
| Rwork | 0.219 |
| R-free | 0.25200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1prg |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.320 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.016 | |
| Number of reflections | 12556 | 1238 |
| <I/σ(I)> | 19.73 | |
| Completeness [%] | 96.6 | |
| Redundancy | 2 | |
| CC(1/2) | 0.914 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 290 | NH4 acetate 0.3M, Hepes 0.1M pH 7.5, di-sodium tartrate 0.8 M |
