6FZL
Crystal structure of human transthyretin double mutant K35T/T119M
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALBA BEAMLINE XALOC |
Synchrotron site | ALBA |
Beamline | XALOC |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-07-07 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97949 |
Spacegroup name | P 21 2 21 |
Unit cell lengths | 43.056, 63.580, 85.957 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.979 - 1.446 |
R-factor | 0.1819 |
Rwork | 0.181 |
R-free | 0.19150 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1f41 |
RMSD bond length | 0.016 |
RMSD bond angle | 1.691 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER (1.7.3-928) |
Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 63.580 | 63.580 | 1.451 |
High resolution limit [Å] | 1.446 | 6.711 | 1.446 |
Rmerge | 0.046 | 0.023 | 0.849 |
Rmeas | 0.050 | 0.025 | 0.927 |
Rpim | 0.020 | 0.011 | 0.369 |
Total number of observations | 267716 | ||
Number of reflections | 41465 | 512 | 402 |
<I/σ(I)> | 19.7 | ||
Completeness [%] | 96.6 | 100 | 96.6 |
Redundancy | 6.5 | 5.7 | 6.2 |
CC(1/2) | 0.999 | 0.999 | 0.734 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | 7% glycerol, 1.3 M sodium citrate, pH 5.5 |