6FYW
Structure of B/Brisbane/60/2008 Influenza Hemagglutinin in complex with SD83
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-15 |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 1.0331 |
| Spacegroup name | I 21 3 |
| Unit cell lengths | 189.040, 189.040, 189.040 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.260 - 2.200 |
| R-factor | 0.1933 |
| Rwork | 0.193 |
| R-free | 0.21790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4fqm |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.612 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_2614: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rpim | 0.060 | 0.440 |
| Number of reflections | 56754 | 5669 |
| <I/σ(I)> | 9.9 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.1 | 5.1 |
| CC(1/2) | 1.000 | 0.620 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 277 | 0.1 M MES, pH 6.5 and 10% PEG 20,000 |
