6FYT
Structure of H1 (A/solomon Islands/3/06) Influenza Hemagglutinin in complex with SD38
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-10-20 |
Detector | MARRESEARCH |
Wavelength(s) | 1.03316 |
Spacegroup name | P 63 |
Unit cell lengths | 107.540, 107.540, 198.330 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.268 - 2.800 |
R-factor | 0.2021 |
Rwork | 0.200 |
R-free | 0.23460 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.593 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((dev_2614: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rpim | 0.060 | 0.530 |
Number of reflections | 31685 | 3141 |
<I/σ(I)> | 10.3 | 2 |
Completeness [%] | 99.0 | 98 |
Redundancy | 3.5 | 3.5 |
CC(1/2) | 1.000 | 0.690 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8 | 277 | 0.2 M calcium acetate, 10 % PEG800 and o.1 M imidazole pH 8.0 |