6FY5
Crystal structure of the macro domain of human macroh2a2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-05-07 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.97565 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 57.750, 41.370, 84.150 |
Unit cell angles | 90.00, 97.05, 90.00 |
Refinement procedure
Resolution | 19.680 - 1.650 |
R-factor | 0.216 |
Rwork | 0.215 |
R-free | 0.24400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1zr3 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.120 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.690 | 1.690 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmeas | 0.060 | 1.100 |
Number of reflections | 46778 | 2724 |
<I/σ(I)> | 13.15 | 1.25 |
Completeness [%] | 97.3 | 92.6 |
Redundancy | 5.4 | 3.4 |
CC(1/2) | 0.999 | 0.450 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 298 | 12.5% (w/v) PEG 1,000, 0.2 M sodium acetate, 0.1 M Mes pH 6.5 |