6FWT

Crystal structure of human wild type beta-1,4-galactosyltransferase-1 (B4GalT1) in apo-open monomeric form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-02-22
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97950
Spacegroup name	P 2 21 21
Unit cell lengths	42.278, 43.716, 144.348
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.278 - 1.845
R-factor	0.1685
Rwork	0.166
R-free	0.21800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ee3
RMSD bond length	0.010
RMSD bond angle	1.012
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.280	1.911
High resolution limit [Å]	1.845	1.845
Rmerge	0.110	0.967
Rmeas	0.120	1.126
Rpim	0.048	0.568
Number of reflections	23816
<I/σ(I)>	11.47
Completeness [%]	99.8
Redundancy	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M Potassium Nitrate, 20% (w/v) PEG 3350