6FVM

Mutant DNA polymerase sliding clamp from Escherichia coli with bound P7 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-02-26
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.00
Spacegroup name	P 1
Unit cell lengths	34.336, 78.653, 80.182
Unit cell angles	113.26, 97.82, 95.97

Refinement procedure

Resolution	66.302 - 1.631
R-factor	0.1821
Rwork	0.180
R-free	0.21920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ok7
RMSD bond length	0.008
RMSD bond angle	0.966
Data reduction software	XDS
Data scaling software	autoPROC
Phasing software	MOLREP
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	72.266	72.266	1.659
High resolution limit [Å]	1.631	4.426	1.631
Rmerge	0.084	0.035	1.191
Rmeas	0.092	0.038	1.418
Rpim	0.035	0.014	0.751
Number of reflections	64980	4688	77
<I/σ(I)>	13.2	41	1.1
Completeness [%]	87.6	99.9	31.6
Redundancy	6.6	6.9	3.4
CC(1/2)	0.999	0.999	0.308

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	MES 50mM pH 6, CaCl2 50mM, PEG400 28%+ Hampton Research PEG Ion kit B3 (1 microliter): 0.2M lithium nitrate, 20% PEG 3350 pH 7.1