6FVL

DNA polymerase sliding clamp from Escherichia coli with bound P7 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-02-26
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.0
Spacegroup name	P 1
Unit cell lengths	72.900, 79.480, 85.317
Unit cell angles	65.83, 75.16, 75.79

Refinement procedure

Resolution	57.055 - 1.975
R-factor	0.191
Rwork	0.189
R-free	0.23320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ok7
RMSD bond length	0.007
RMSD bond angle	1.026
Data reduction software	XDS
Data scaling software	autoPROC
Phasing software	MOLREP
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	57.055	57.055	2.010
High resolution limit [Å]	1.975	5.361	1.975
Rmerge	0.089	0.031	0.706
Rmeas	0.105	0.037	0.993
Rpim	0.055	0.027	0.697
Number of reflections	76533	5808	192
<I/σ(I)>	11	32.6	1.1
Completeness [%]	87.6	99.2	25.9
Redundancy	3.5	3.5
CC(1/2)	0.997	0.999	0.234

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	MES 50mM pH 6, CaCl2 50mM PEG400 30% (1 microliter) + Hampton Research PEG Ion kit E6 (1 microliter): 0.2M sodium malonate pH6, 20% PEG 3350