6FVG
The Structure of CK2alpha with CCh507 bound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-06-25 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.92818 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 60.183, 45.931, 63.706 |
Unit cell angles | 90.00, 111.22, 90.00 |
Refinement procedure
Resolution | 56.100 - 1.600 |
R-factor | 0.208 |
Rwork | 0.206 |
R-free | 0.22900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5cu6 |
RMSD bond length | 0.010 |
RMSD bond angle | 0.920 |
Data reduction software | DIALS |
Data scaling software | Aimless (0.5.26) |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 56.100 | 56.100 | 1.640 |
High resolution limit [Å] | 1.600 | 7.160 | 1.600 |
Rmerge | 0.049 | 0.042 | 0.727 |
Rmeas | 0.058 | 0.049 | 0.866 |
Rpim | 0.031 | 0.026 | 0.466 |
Number of reflections | 43028 | ||
<I/σ(I)> | 9.2 | ||
Completeness [%] | 99.9 | 99.6 | 99.8 |
Redundancy | 3.3 | 3.5 | 3.3 |
CC(1/2) | 0.997 | 0.992 | 0.745 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 112.5mM Mes pH 6.5, 35% glycerol ethoxylate, 180 mM ammonium acetate |