6FVE

Macrophage Migration Inhibitory Factor (MIF) with Covalently Bound FITC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-10
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.987
Spacegroup name	P 21 21 21
Unit cell lengths	46.783, 70.644, 89.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	14.970 - 1.410
R-factor	0.13647
Rwork	0.134
R-free	0.18926
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3l5v
RMSD bond length	0.012
RMSD bond angle	1.593
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.440
High resolution limit [Å]	1.410	1.410
Rmerge	0.068	0.783
Number of reflections	57778
<I/σ(I)>	13.84
Completeness [%]	99.4
Redundancy	11.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298	Ammonium Sulfate, 0.1M Hepes pH 7.0