6FUJ
Complement factor D in complex with the inhibitor N-(3'-(aminomethyl)-[1,1'-biphenyl]-3-yl)-3-methylbutanamide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2008-04-20 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1.00 |
Spacegroup name | C 2 2 2 |
Unit cell lengths | 116.662, 210.535, 143.521 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 71.760 - 2.250 |
R-factor | 0.21892 |
Rwork | 0.218 |
R-free | 0.24388 |
RMSD bond length | 0.015 |
RMSD bond angle | 1.787 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 83.050 | 2.320 |
High resolution limit [Å] | 2.250 | 2.250 |
Number of reflections | 83798 | |
<I/σ(I)> | 10 | |
Completeness [%] | 100.0 | |
Redundancy | 6.14 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 22-25% PEG3350, 100 mM HEPES pH 7.5, 2 mM inhibitor |