6FQZ
Plasmodium falciparum 6-phosphogluconate dehydrogenase in its apo form, in complex with its cofactor NADP+ and in complex with its substrate 6-phosphogluconate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-17 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 99.660, 99.660, 180.780 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.409 - 1.900 |
| R-factor | 0.1984 |
| Rwork | 0.198 |
| R-free | 0.22930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6fqx |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.497 |
| Data reduction software | XDS (2017) |
| Data scaling software | XSCALE (2017) |
| Phasing software | PHENIX (svn 7924) |
| Refinement software | PHENIX ((dev_2645: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.410 | 1.968 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.160 | |
| Rmeas | 0.170 | |
| Rpim | 0.053 | 0.990 |
| Number of reflections | 82520 | 8165 |
| <I/σ(I)> | 10.28 | 0.68 |
| Completeness [%] | 100.0 | 99.93 |
| Redundancy | 10 | 10.3 |
| CC(1/2) | 0.998 | 0.330 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 6PG-Pf6PGD crystals were crystallized with 24% PEG 1500 and 20% glycerol. |
