6FQP
Crystal structure of TALE homeobox domain transcription factor TGIF1 with its consensus DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-05-10 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.972422 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 50.851, 67.248, 102.315 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.537 - 2.420 |
R-factor | 0.2069 |
Rwork | 0.205 |
R-free | 0.24090 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4xrm |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 67.250 | 2.510 |
High resolution limit [Å] | 2.420 | 2.420 |
Rmerge | 0.028 | 0.232 |
Number of reflections | 13814 | |
<I/σ(I)> | 17.3 | |
Completeness [%] | 98.9 | 98.7 |
Redundancy | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.1M sodium acetate trihydrate pH 4.5, 30% v/v PEG 1500 |