6FQK

GluA2(flop) S729C ligand binding core dimer bound to ZK200775 at 1.98 Angstrom resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-18
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9795
Spacegroup name	P 61
Unit cell lengths	106.696, 106.696, 100.394
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	53.348 - 1.980
R-factor	0.219368406855
Rwork	0.217
R-free	0.25573
RMSD bond length	0.009
RMSD bond angle	1.154
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.348	2.010
High resolution limit [Å]	1.980	1.980
Rmeas	0.060
Rpim	0.024
Number of reflections	43458
<I/σ(I)>	17.2
Completeness [%]	100.0
Redundancy	6.5
CC(1/2)	0.997

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		287	0.2M Ammonium Chloride, 20% PEG 3350, 10microM ZK200775.