6FPY

Inter-alpha-inhibitor heavy chain 1, wild type

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-04-27
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9282
Spacegroup name	P 42
Unit cell lengths	158.767, 158.767, 65.371
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	79.383 - 2.339
R-factor	0.2328
Rwork	0.231
R-free	0.26120
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	Aimless (0.5.32)
Phasing software	SOLVE
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	79.380	79.380	2.400
High resolution limit [Å]	2.340	10.460	2.340
Rmerge	0.144	0.051	1.110
Rmeas	0.157	0.056	1.205
Rpim	0.060	0.021	0.464
Total number of observations	455822	5835	33758
Number of reflections	69124	846	5063
<I/σ(I)>	8	23.2	1.8
Completeness [%]	99.9	99.8	100
Redundancy	6.6	6.9	6.7
CC(1/2)	0.994	0.998	0.496

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	293	100mM HEPES, pH 7.5, 100mM Sodium acetate, 10% PEG8K, 20% Glycerol