6FPK
Co-translational folding intermediate dictates membrane targeting of the signal recognition particle (SRP)- receptor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-12-15 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.953723 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 56.096, 82.555, 29.813 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.400 - 1.950 |
| R-factor | 0.21406 |
| Rwork | 0.212 |
| R-free | 0.23380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qy9 |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.353 |
| Data reduction software | MOSFLM |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.400 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Number of reflections | 5318 | 519 |
| <I/σ(I)> | 19.66 | 3.6 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 7.1 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 21% MPD 0.05M NaCacodylate pH=6.2 |
