6FNF
Crystal Structure of Ephrin A2 (EphA2) Receptor Protein Kinase with NVP-BHG712
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-11-10 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.97625 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 32.230, 104.796, 39.957 |
Unit cell angles | 90.00, 108.31, 90.00 |
Refinement procedure
Resolution | 35.669 - 1.556 |
R-factor | 0.2338 |
Rwork | 0.235 |
R-free | 0.27270 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5i9u |
RMSD bond length | 0.013 |
RMSD bond angle | 1.248 |
Data reduction software | XDS (XDSapp 2.0) |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.930 | 1.630 |
High resolution limit [Å] | 1.540 | 1.540 |
Rmeas | 0.200 | |
Number of reflections | 35632 | 4526 |
<I/σ(I)> | 5.93 | 0.22 |
Completeness [%] | 95.5 | 75.9 |
Redundancy | 5.99 | 4.97 |
CC(1/2) | 0.996 | 0.127 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 37.5 % MPD/PEG1000/PEG3350 (MD), 0.1 M Amino Acids Mix (MD), 0.1 M Tris pH 7.5 |