6FM1

Deoxyguanylosuccinate synthase (DgsS) quaternary structure with ATPanddGMP at 2.3 Angstrom resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2016-12-23
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.54179
Spacegroup name	P 41 21 2
Unit cell lengths	90.260, 90.260, 188.440
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.970 - 2.350
R-factor	0.185
Rwork	0.183
R-free	0.23200
RMSD bond length	0.010
RMSD bond angle	1.170
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.000	2.440
High resolution limit [Å]	2.300	2.300
Number of reflections	35274
<I/σ(I)>	12.58
Completeness [%]	99.7
Redundancy	13.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		290	0.5 M KCl 10 %w/v Glycerol 12 %w/v PEG 8K