6FLN
Crystal structure of the human TRIM25 coiled-coil and PRYSPRY domains
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-11-16 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 1.072 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 89.920, 89.920, 827.090 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 137.850 - 3.600 |
R-factor | 0.28877 |
Rwork | 0.287 |
R-free | 0.31844 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Separate coiled-coil (PDB:4LTB) and PRYSPRY (PDB:6FLM) domains |
RMSD bond length | 0.008 |
RMSD bond angle | 1.051 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 137.850 | 3.690 |
High resolution limit [Å] | 3.600 | 3.600 |
Rmerge | 0.136 | 2.380 |
Rmeas | 0.151 | 2.640 |
Number of reflections | 24552 | |
<I/σ(I)> | 8.18 | |
Completeness [%] | 99.5 | 98.5 |
Redundancy | 5.36 | 5.51 |
CC(1/2) | 0.999 | 0.569 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | Protein at 10 mg/ml was mixed in 2:1 ratio with mother liquor containing 0.2 M sodium formate, 14% PEG 3350 and 0.1 M bis-Tris pH 6.5. |