6FLM
Crystal structure of the human TRIM25 PRYSPRY domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-11-14 |
Detector | MARRESEARCH |
Wavelength(s) | 0.8726 |
Spacegroup name | P 3 2 1 |
Unit cell lengths | 152.750, 152.750, 68.960 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.735 - 2.009 |
R-factor | 0.167 |
Rwork | 0.165 |
R-free | 0.20610 |
Structure solution method | SIRAS |
RMSD bond length | 0.005 |
RMSD bond angle | 0.707 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHARP |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.735 | 2.100 |
High resolution limit [Å] | 2.009 | 2.010 |
Rmerge | 0.124 | 0.336 |
Rmeas | 0.135 | 0.412 |
Number of reflections | 52640 | |
<I/σ(I)> | 11.42 | 2.36 |
Completeness [%] | 84.1 | |
Redundancy | 5.34 | |
CC(1/2) | 0.996 | 0.837 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 15 mg/ml protein mixed in 1:1 ratio with reservoir containing 0.1 M bis-TRIS pH 6.5 and 2M ammonium sulphate. |