6FL1
Crystal structure of the complex between the Lactococcus lactis FPG mutant T221P and a Fapy-dG containing DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-02-04 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.95373 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 91.369, 91.369, 141.673 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.735 - 1.600 |
| R-factor | 0.1639 |
| Rwork | 0.163 |
| R-free | 0.17830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xc8 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.989 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.740 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.050 | 0.462 |
| Number of reflections | 78211 | 10201 |
| <I/σ(I)> | 21.3 | 3.5 |
| Completeness [%] | 98.4 | 90 |
| Redundancy | 7 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | HEPES, SODIUM CITRATE |
