6FKO
Deoxyguanylosuccinate synthase (DgsS) quaternary structure with ATP, dGMP, hadacidin at 2.1 Angstrom resolution
Replaces: 6FJRExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-06-09 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.980097 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 91.970, 91.970, 192.750 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.710 - 2.100 |
| R-factor | 0.191 |
| Rwork | 0.189 |
| R-free | 0.22200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.080 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.188 | 2.220 |
| High resolution limit [Å] | 2.106 | 2.106 |
| Number of reflections | 49180 | |
| <I/σ(I)> | 8.4 | |
| Completeness [%] | 99.5 | |
| Redundancy | 5.91 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 291 | 0.2 M (NH4)2SO4 0.1 M Na3 Cit 5.6 pH 25 %w/v PEG 4K |
