6FKM

Drosophila Plexin A in complex with Semaphorin 1b

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-07-03
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.072
Spacegroup name	C 2 2 21
Unit cell lengths	130.940, 195.090, 124.840
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	76.864 - 2.960
R-factor	0.1891
Rwork	0.186
R-free	0.24620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3oky 6fkk
RMSD bond length	0.005
RMSD bond angle	0.755
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX ((1.13rc2_2986: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	76.864	3.070
High resolution limit [Å]	2.960	2.960
Rmerge	0.174	0.634
Number of reflections	31584
<I/σ(I)>	8.86
Completeness [%]	93.8
Redundancy	4.6
CC(1/2)	0.990

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	0.1 M HEPES (pH 7.0) and 8% (w/v) PEG 8000