6FKJ
Tubulin-TUB075 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-10-02 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.00002 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 104.670, 157.562, 180.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.445 - 2.148 |
| R-factor | 0.1763 |
| Rwork | 0.175 |
| R-free | 0.20640 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 4o2b |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.719 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((dev_2863: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.450 | 2.210 |
| High resolution limit [Å] | 2.148 | 2.150 |
| Rmerge | 0.114 | 2.429 |
| Rmeas | 0.118 | 2.577 |
| Rpim | 0.034 | 0.884 |
| Number of reflections | 161351 | 11396 |
| <I/σ(I)> | 16.6 | 0.8 |
| Completeness [%] | 99.5 | 96.3 |
| Redundancy | 12.5 | 8.9 |
| CC(1/2) | 0.999 | 0.300 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.7 | 294 | 5% PEG 4K, 12% glycerol, 30 mM MgCl2, 30 mM CaCl2, 100 mM MES/Imidazole |
