6FKG
Crystal structure of the M.tuberculosis MbcT-MbcA toxin-antitoxin complex.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-07-25 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 2.48, 0.9765 |
Spacegroup name | P 63 |
Unit cell lengths | 105.311, 105.311, 108.712 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 9.988 - 1.800 |
R-factor | 0.1646 |
Rwork | 0.162 |
R-free | 0.21110 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 0.988 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHELXCD |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 9.990 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.056 | 1.364 |
Number of reflections | 62157 | 5639 |
<I/σ(I)> | 21.6 | 1.5 |
Completeness [%] | 98.8 | 90.21 |
Redundancy | 10 | 7.5 |
CC(1/2) | 0.999 | 0.497 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 288.15 | 0.2 M ammonium sulphate, 0.1 M tri-sodium citrate pH 5.6 and 25 % PEG 4000 |