6FKG

Crystal structure of the M.tuberculosis MbcT-MbcA toxin-antitoxin complex.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-07-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	2.48, 0.9765
Spacegroup name	P 63
Unit cell lengths	105.311, 105.311, 108.712
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	9.988 - 1.800
R-factor	0.1646
Rwork	0.162
R-free	0.21110
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	0.988
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELXCD
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	9.990	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.056	1.364
Number of reflections	62157	5639
<I/σ(I)>	21.6	1.5
Completeness [%]	98.8	90.21
Redundancy	10	7.5
CC(1/2)	0.999	0.497

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		288.15	0.2 M ammonium sulphate, 0.1 M tri-sodium citrate pH 5.6 and 25 % PEG 4000