6FKA
Crystal structure of N2C/D282C stabilized opsin bound to RS11
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-12-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 244.124, 244.124, 111.201 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.737 - 2.700 |
| R-factor | 0.2102 |
| Rwork | 0.210 |
| R-free | 0.22150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4j4q |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.687 |
| Data reduction software | XDS ((VERSION Oct 15) |
| Data scaling software | Aimless |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.740 | 2.560 |
| High resolution limit [Å] | 2.460 | 2.460 |
| Rmerge | 0.092 | 0.957 |
| Number of reflections | 45835 | |
| <I/σ(I)> | 16.2 | 0.7 |
| Completeness [%] | 99.8 | 99.7 |
| Redundancy | 11.45 | 11.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | see publication |
