6FJD

Human KIBRA C2 domain mutant C771A in complex with phosphatidylinositol 4,5-bisphosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2014-10-31
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.54
Spacegroup name	P 61 2 2
Unit cell lengths	83.404, 83.404, 208.254
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.470 - 2.898
R-factor	0.2049
Rwork	0.203
R-free	0.24170
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2z0u
RMSD bond length	0.010
RMSD bond angle	1.012
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	BALBES
Refinement software	PHENIX ((1.12rc0_2797: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.950
High resolution limit [Å]	2.898	2.898
Rmerge	0.134	0.606
Number of reflections	10159
<I/σ(I)>	11.5	2.099
Completeness [%]	99.4	98.4
Redundancy	5.8	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	0.1M Tris pH 8.0, 1.5M (NH4)2SO4