6FII

Tubulin-Spongistatin complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-04-21
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.00004
Spacegroup name	P 21 21 21
Unit cell lengths	105.670, 159.920, 181.010
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.168 - 2.405
R-factor	0.1703
Rwork	0.168
R-free	0.21600
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	4i4t
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX ((dev_2875: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.200	2.470
High resolution limit [Å]	2.400	2.400
Rmerge	0.167	2.734
Rmeas	0.172	2.826
Number of reflections	115484	8272
<I/σ(I)>	15.8	1.06
Completeness [%]	99.8	97.5
Redundancy	16.7	15.6
CC(1/2)	0.999	0.360

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.7	294	10 % PEG 4k, 16 % glycerol, 30 mM MgCl2, 30 mM CaCl2, and 100 mM 2-(N-morpholino)ethanesulfonic acid/imidazole