6FIB
Structure of human 4-1BB ligand
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-11-25 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.99993 |
Spacegroup name | P 62 |
Unit cell lengths | 119.592, 119.592, 104.350 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 103.570 - 2.700 |
R-factor | 0.169 |
Rwork | 0.166 |
R-free | 0.22100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2x29 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.290 |
Data reduction software | XDS |
Data scaling software | SADABS |
Phasing software | PHASER |
Refinement software | BUSTER (2.11.7) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 103.600 | 3.042 |
High resolution limit [Å] | 2.697 | 2.697 |
Number of reflections | 14507 | 725 |
<I/σ(I)> | 21.6 | |
Completeness [%] | 91.4 | 64.4 |
Redundancy | 11.4 | 11 |
CC(1/2) | 1.000 | 0.703 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.1M Tris, pH 8.5, 20% PEG1000, 0.009M BaCl2 |