6FI9
Crystal Structure of a zinc-responsive MarR family member, Lactococcus lactis ZitR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-02-10 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.28189 |
| Spacegroup name | P 41 |
| Unit cell lengths | 128.520, 128.520, 88.140 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.150 - 2.800 |
| R-factor | 0.237 |
| Rwork | 0.236 |
| R-free | 0.25100 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.020 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.110 | 3.170 |
| High resolution limit [Å] | 3.090 | 3.090 |
| Rmerge | 0.067 | |
| Rmeas | 0.095 | |
| Number of reflections | 26339 | 1860 |
| <I/σ(I)> | 12.7 | 1 |
| Completeness [%] | 99.6 | 96 |
| Redundancy | 8.9 | |
| CC(1/2) | 0.999 | 0.597 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | 0.1 M sodium citrate tribasic dihydrate 30 % polyethyleneglycol 4.000 |
