6FHZ
Inward-facing conformation of a multidrug resistance MATE family transporter of the MOP superfamily.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-02-05 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 107.120, 134.310, 69.710 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.803 - 2.800 |
R-factor | 0.2306 |
Rwork | 0.229 |
R-free | 0.27400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4mlb |
RMSD bond length | 0.001 |
RMSD bond angle | 0.360 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.700 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.223 | |
Rmeas | 0.238 | |
Rpim | 0.082 | 0.749 |
Number of reflections | 12731 | 1263 |
<I/σ(I)> | 8.8 | 0.9 |
Completeness [%] | 99.8 | 99.8 |
Redundancy | 8.3 | 5.5 |
CC(1/2) | 0.996 | 0.296 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 5 | 295 | 30% PEG 600, 100 mM Na3C6H5O7-C6H8O7, 100 mM NaCl, 100 mM MgCl2 |