6FHZ
Inward-facing conformation of a multidrug resistance MATE family transporter of the MOP superfamily.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-05 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 107.120, 134.310, 69.710 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.803 - 2.800 |
| R-factor | 0.2306 |
| Rwork | 0.229 |
| R-free | 0.27400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4mlb |
| RMSD bond length | 0.001 |
| RMSD bond angle | 0.360 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.700 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.223 | |
| Rmeas | 0.238 | |
| Rpim | 0.082 | 0.749 |
| Number of reflections | 12731 | 1263 |
| <I/σ(I)> | 8.8 | 0.9 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 8.3 | 5.5 |
| CC(1/2) | 0.996 | 0.296 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 5 | 295 | 30% PEG 600, 100 mM Na3C6H5O7-C6H8O7, 100 mM NaCl, 100 mM MgCl2 |
