6FGO

Fc in complex with engineered calcium binding domain Z

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-24
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.0000
Spacegroup name	P 21 21 2
Unit cell lengths	90.229, 120.296, 126.330
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.529 - 2.500
R-factor	0.2048
Rwork	0.203
R-free	0.23190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5u4y
RMSD bond length	0.003
RMSD bond angle	0.575
Data reduction software	XDS
Data scaling software	SCALA (3.3.21)
Phasing software	PHASER
Refinement software	PHENIX (1.12-2829)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.530	46.529	2.640
High resolution limit [Å]	2.500	7.910	2.500
Rmerge		0.031	0.420
Rmeas	0.067	0.035	0.474
Rpim	0.030	0.016	0.213
Number of reflections	47055	1542	6882
<I/σ(I)>	13.8	18.4	1.8
Completeness [%]	98.0	93.9	99.3
Redundancy	4.4	4.2	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	291	24 % PEG3350, 0.1 M LiCl2, 0.1 M MES pH 6.0