6FGB
Human FcRn extra-cellular domain complexed with Fab fragment of Rozanolixizumab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 77 |
| Detector technology | PIXEL |
| Collection date | 2012-02-27 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 150.100, 150.100, 89.150 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.900 |
| R-factor | 0.23429 |
| Rwork | 0.232 |
| R-free | 0.28386 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3m17 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.338 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.060 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.092 | |
| Number of reflections | 24590 | |
| <I/σ(I)> | 13.4 | |
| Completeness [%] | 99.9 | |
| Redundancy | 6.65 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 291.15 | 0.1M Sodium citrate pH 5.5, 11% PEG6000 |
