6FG2
CRYSTAL STRUCTURE OF FAB OF NATALIZUMAB IN COMPLEX WITH FAB OF NAA84.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE MASSIF-1 |
Synchrotron site | ESRF |
Beamline | MASSIF-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-08-25 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 0.966 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 105.404, 66.619, 199.502 |
Unit cell angles | 90.00, 98.97, 90.00 |
Refinement procedure
Resolution | 55.210 - 2.788 |
R-factor | 0.239 |
Rwork | 0.238 |
R-free | 0.26300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4irz 3S37 and 4OD2 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.030 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | BUSTER (2.11.7) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 197.060 | 3.143 |
High resolution limit [Å] | 2.788 | 2.788 |
Rmerge | 0.143 | |
Number of reflections | 40020 | |
<I/σ(I)> | 6.3 | |
Completeness [%] | 98.6 | |
Redundancy | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 292 | MES 100mM - PEG 400 35% - NaCl 200mM - MPD 4% - pH6 |