6FF8
Crystal structure of uncomplexed Rab32 in the active GTP-bound state at 2.13 Angstrom resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-09-16 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9801 |
Spacegroup name | P 43 |
Unit cell lengths | 53.658, 53.658, 129.784 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.352 - 2.130 |
R-factor | 0.1923 |
Rwork | 0.191 |
R-free | 0.22320 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4cym |
RMSD bond length | 0.018 |
RMSD bond angle | 1.922 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.360 | 2.190 |
High resolution limit [Å] | 2.130 | 2.130 |
Rmerge | 0.891 | |
Rmeas | 1.020 | |
Rpim | 0.488 | |
Number of reflections | 20447 | 1646 |
<I/σ(I)> | 12.4 | 1.5 |
Completeness [%] | 99.3 | 99.7 |
Redundancy | 4.2 | 4.2 |
CC(1/2) | 0.588 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | O.2M sodium potassium tartrate, 0.1M Bis-Tris propane pH 8.5, 20% w/v PEG 3350 |