6FF8

Crystal structure of uncomplexed Rab32 in the active GTP-bound state at 2.13 Angstrom resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	CCD
Collection date	2014-09-16
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9801
Spacegroup name	P 43
Unit cell lengths	53.658, 53.658, 129.784
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.352 - 2.130
R-factor	0.1923
Rwork	0.191
R-free	0.22320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4cym
RMSD bond length	0.018
RMSD bond angle	1.922
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.360	2.190
High resolution limit [Å]	2.130	2.130
Rmerge		0.891
Rmeas		1.020
Rpim		0.488
Number of reflections	20447	1646
<I/σ(I)>	12.4	1.5
Completeness [%]	99.3	99.7
Redundancy	4.2	4.2
CC(1/2)		0.588

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	O.2M sodium potassium tartrate, 0.1M Bis-Tris propane pH 8.5, 20% w/v PEG 3350