6FF3
Crystal structure of Drosophila neural ectodermal development factor Imp-L1 with Human IGF-I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-02-28 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 82.030, 94.150, 48.360 |
| Unit cell angles | 90.00, 95.26, 90.00 |
Refinement procedure
| Resolution | 48.160 - 2.570 |
| R-factor | 0.23199 |
| Rwork | 0.229 |
| R-free | 0.28520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4cbp |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.676 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.160 | 2.640 |
| High resolution limit [Å] | 2.570 | 2.570 |
| Rmerge | 0.093 | 0.783 |
| Rpim | 0.059 | 0.470 |
| Number of reflections | 10666 | |
| <I/σ(I)> | 3.9 | |
| Completeness [%] | 96.4 | 97.6 |
| Redundancy | 3.9 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 291 | cp 10 mg/ml, 4-8% w/v PEG 6K, 5-20 mM MgCl2, 5 mM SB12, 0.1 M TRIS pH 7.5 |
