6FEY
Crystal structure of Drosophila neural ectodermal development factor Imp-L2 with Drosophila DILP5 insulin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-03-19 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.91500 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 150.834, 150.834, 125.321 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.600 - 3.480 |
R-factor | 0.26548 |
Rwork | 0.261 |
R-free | 0.34456 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4cbp |
RMSD bond length | 0.011 |
RMSD bond angle | 1.734 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.600 | 3.670 |
High resolution limit [Å] | 3.480 | 3.480 |
Rmerge | 0.165 | 1.170 |
Rpim | 0.089 | 0.630 |
Number of reflections | 17857 | |
<I/σ(I)> | 8 | |
Completeness [%] | 98.8 | |
Redundancy | 4.3 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 291 | Cp 10 mg/ml, 8-10% w/v PEG 4k or 6K, 20 mM MgCl2, 0.1 M HEPES pH 6.8-7.5 |