6FEL
Structure of 14-3-3 gamma in complex with CaMKK2 14-3-3 binding motif Ser511
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | LIQUID ANODE |
| Source details | Excillum MetalJet D2+ 70 kV |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-08-30 |
| Detector | Bruker PHOTON II |
| Wavelength(s) | 1.3418 |
| Spacegroup name | H 3 |
| Unit cell lengths | 115.734, 115.734, 203.963 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 26.820 - 2.840 |
| R-factor | 0.2304 |
| Rwork | 0.228 |
| R-free | 0.27990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b05 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.550 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.820 | 2.940 |
| High resolution limit [Å] | 2.840 | 2.840 |
| Rmeas | 0.260 | 0.970 |
| Number of reflections | 22801 | 2424 |
| <I/σ(I)> | 11.19 | 2.26 |
| Completeness [%] | 94.4 | 99.4 |
| Redundancy | 10.85 | 10.81 |
| CC(1/2) | 0.991 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.6 | 291.15 | sodium citrate, potassium sodium tartrate, and ammonium sulfate |
