6FDK
Structure of Chlamydia trachomatis effector protein Cdu1 bound to ubiquitin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-11-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.980 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.815, 56.937, 60.695 |
| Unit cell angles | 90.00, 104.86, 90.00 |
Refinement procedure
| Resolution | 46.220 - 1.600 |
| R-factor | 0.17122 |
| Rwork | 0.170 |
| R-free | 0.20333 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5b5q |
| RMSD bond length | 0.030 |
| RMSD bond angle | 2.530 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.220 | 1.657 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmeas | 0.072 | |
| Rpim | 0.027 | 0.647 |
| Number of reflections | 41326 | 4140 |
| <I/σ(I)> | 17.06 | 1.32 |
| Completeness [%] | 98.9 | 99.9 |
| Redundancy | 6.7 | 7 |
| CC(1/2) | 0.999 | 0.561 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 0.1 M MES pH 6.5, 12% PEG 20000 |
